Crystallization of a proteolyzed form of the horse pancreatic lipase‐related protein 2: structural basis for the specific detergent requirement
Identifieur interne : 000079 ( France/Analysis ); précédent : 000078; suivant : 000080Crystallization of a proteolyzed form of the horse pancreatic lipase‐related protein 2: structural basis for the specific detergent requirement
Auteurs : José M. Manche O [Espagne, France] ; Sandrine Jayne [Espagne, France] ; Brigritte Kerfelec [Espagne, France] ; Catherine Chapus [Espagne, France] ; Isabelle Crenon [Espagne, France] ; Juan A. Hermoso [Espagne, France]Source :
- Acta Crystallographica Section D [ 1399-0047 ] ; 2004-11.
English descriptors
- KwdEn :
Abstract
Horse pancreatic lipase‐related proteins PLRP1 and PLRP2 are produced by the pancreas together with pancreatic lipase (PL). Sequence‐comparison analyses reveal that the three proteins possess the same two‐domain organization: an N‐terminal catalytic domain and a C‐terminal domain, which in PL is involved in colipase binding. Nevertheless, despite the high level of sequence identity found, they exhibit distinct enzymatic properties. The intrinsic sensitivity of the peptide bond between Ser245 and Thr246 within the flap region of PLRP2 to proteolytic cleavage probably complicates PLRP2 crystallization since, as shown here, this proteolyzed form of PLRP2 is only crystallized after specific detergent stabilization of this region. This has been performed by the hanging‐drop vapour‐diffusion method at 291 K and exclusively in the presence of N,N‐dimethyldecylamine‐β‐oxide (DDAO). However, most crystals (>95%) are highly twinned and diffract poorly (to ∼7–5 Å resolution). Diffraction‐quality trigonal crystals have unit‐cell parameters a = b = 128.4, c = 85.8 Å and belong to space group P3221. A 2.9 Å native data set was collected at ESRF on beamline ID14‐2 with an Rmerge of 12.7%. Preliminary structural analysis provides a structural basis for the specific roles of DDAO.
Url:
DOI: 10.1107/S0907444904024229
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 000736
- to stream Istex, to step Curation: 000586
- to stream Istex, to step Checkpoint: 001752
- to stream Main, to step Merge: 001E75
- to stream Main, to step Curation: 001E40
- to stream Main, to step Exploration: 001E40
- to stream France, to step Extraction: 000079
Links to Exploration step
ISTEX:8DAD7EA4E8046AC3148EB40846CE1E985B53AC5ELe document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Crystallization of a proteolyzed form of the horse pancreatic lipase‐related protein 2: structural basis for the specific detergent requirement</title><author><name sortKey="Manche O, Jose M" sort="Manche O, Jose M" uniqKey="Manche O J" first="José M." last="Manche O">José M. Manche O</name></author><author><name sortKey="Jayne, Sandrine" sort="Jayne, Sandrine" uniqKey="Jayne S" first="Sandrine" last="Jayne">Sandrine Jayne</name></author><author><name sortKey="Kerfelec, Brigritte" sort="Kerfelec, Brigritte" uniqKey="Kerfelec B" first="Brigritte" last="Kerfelec">Brigritte Kerfelec</name></author><author><name sortKey="Chapus, Catherine" sort="Chapus, Catherine" uniqKey="Chapus C" first="Catherine" last="Chapus">Catherine Chapus</name></author><author><name sortKey="Crenon, Isabelle" sort="Crenon, Isabelle" uniqKey="Crenon I" first="Isabelle" last="Crenon">Isabelle Crenon</name></author><author><name sortKey="Hermoso, Juan A" sort="Hermoso, Juan A" uniqKey="Hermoso J" first="Juan A." last="Hermoso">Juan A. Hermoso</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:8DAD7EA4E8046AC3148EB40846CE1E985B53AC5E</idno><date when="2004" year="2004">2004</date><idno type="doi">10.1107/S0907444904024229</idno><idno type="url">https://api.istex.fr/document/8DAD7EA4E8046AC3148EB40846CE1E985B53AC5E/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">000736</idno><idno type="wicri:Area/Istex/Curation">000586</idno><idno type="wicri:Area/Istex/Checkpoint">001752</idno><idno type="wicri:doubleKey">1399-0047:2004:Manche O J:crystallization:of:a</idno><idno type="wicri:Area/Main/Merge">001E75</idno><idno type="wicri:Area/Main/Curation">001E40</idno><idno type="wicri:Area/Main/Exploration">001E40</idno><idno type="wicri:Area/France/Extraction">000079</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Crystallization of a proteolyzed form of the horse pancreatic lipase‐related protein 2: structural basis for the specific detergent requirement</title><author><name sortKey="Manche O, Jose M" sort="Manche O, Jose M" uniqKey="Manche O J" first="José M." last="Manche O">José M. Manche O</name><affiliation wicri:level="2"><country xml:lang="fr">Espagne</country><wicri:regionArea>Grupo de Cristalografía Macromolecular y Biología Estructural, Instituto Rocasolano, CSIC, Serrano 119, 28006 Madrid</wicri:regionArea><placeName><region nuts="2" type="communauté">Communauté de Madrid</region></placeName></affiliation><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>INSERM‐U476 Nutrition Humaine et Lipides, 18 Avenue Mozart, 13009 Marseille</wicri:regionArea><placeName><region type="region" nuts="2">Provence-Alpes-Côte d'Azur</region><settlement type="city">Marseille</settlement></placeName></affiliation></author><author><name sortKey="Jayne, Sandrine" sort="Jayne, Sandrine" uniqKey="Jayne S" first="Sandrine" last="Jayne">Sandrine Jayne</name><affiliation wicri:level="2"><country xml:lang="fr">Espagne</country><wicri:regionArea>Grupo de Cristalografía Macromolecular y Biología Estructural, Instituto Rocasolano, CSIC, Serrano 119, 28006 Madrid</wicri:regionArea><placeName><region nuts="2" type="communauté">Communauté de Madrid</region></placeName></affiliation><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>INSERM‐U476 Nutrition Humaine et Lipides, 18 Avenue Mozart, 13009 Marseille</wicri:regionArea><placeName><region type="region" nuts="2">Provence-Alpes-Côte d'Azur</region><settlement type="city">Marseille</settlement></placeName></affiliation></author><author><name sortKey="Kerfelec, Brigritte" sort="Kerfelec, Brigritte" uniqKey="Kerfelec B" first="Brigritte" last="Kerfelec">Brigritte Kerfelec</name><affiliation wicri:level="2"><country xml:lang="fr">Espagne</country><wicri:regionArea>Grupo de Cristalografía Macromolecular y Biología Estructural, Instituto Rocasolano, CSIC, Serrano 119, 28006 Madrid</wicri:regionArea><placeName><region nuts="2" type="communauté">Communauté de Madrid</region></placeName></affiliation><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>INSERM‐U476 Nutrition Humaine et Lipides, 18 Avenue Mozart, 13009 Marseille</wicri:regionArea><placeName><region type="region" nuts="2">Provence-Alpes-Côte d'Azur</region><settlement type="city">Marseille</settlement></placeName></affiliation></author><author><name sortKey="Chapus, Catherine" sort="Chapus, Catherine" uniqKey="Chapus C" first="Catherine" last="Chapus">Catherine Chapus</name><affiliation wicri:level="2"><country xml:lang="fr">Espagne</country><wicri:regionArea>Grupo de Cristalografía Macromolecular y Biología Estructural, Instituto Rocasolano, CSIC, Serrano 119, 28006 Madrid</wicri:regionArea><placeName><region nuts="2" type="communauté">Communauté de Madrid</region></placeName></affiliation><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>INSERM‐U476 Nutrition Humaine et Lipides, 18 Avenue Mozart, 13009 Marseille</wicri:regionArea><placeName><region type="region" nuts="2">Provence-Alpes-Côte d'Azur</region><settlement type="city">Marseille</settlement></placeName></affiliation></author><author><name sortKey="Crenon, Isabelle" sort="Crenon, Isabelle" uniqKey="Crenon I" first="Isabelle" last="Crenon">Isabelle Crenon</name><affiliation wicri:level="2"><country xml:lang="fr">Espagne</country><wicri:regionArea>Grupo de Cristalografía Macromolecular y Biología Estructural, Instituto Rocasolano, CSIC, Serrano 119, 28006 Madrid</wicri:regionArea><placeName><region nuts="2" type="communauté">Communauté de Madrid</region></placeName></affiliation><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>INSERM‐U476 Nutrition Humaine et Lipides, 18 Avenue Mozart, 13009 Marseille</wicri:regionArea><placeName><region type="region" nuts="2">Provence-Alpes-Côte d'Azur</region><settlement type="city">Marseille</settlement></placeName></affiliation></author><author><name sortKey="Hermoso, Juan A" sort="Hermoso, Juan A" uniqKey="Hermoso J" first="Juan A." last="Hermoso">Juan A. Hermoso</name><affiliation wicri:level="2"><country xml:lang="fr">Espagne</country><wicri:regionArea>Grupo de Cristalografía Macromolecular y Biología Estructural, Instituto Rocasolano, CSIC, Serrano 119, 28006 Madrid</wicri:regionArea><placeName><region nuts="2" type="communauté">Communauté de Madrid</region></placeName></affiliation><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>INSERM‐U476 Nutrition Humaine et Lipides, 18 Avenue Mozart, 13009 Marseille</wicri:regionArea><placeName><region type="region" nuts="2">Provence-Alpes-Côte d'Azur</region><settlement type="city">Marseille</settlement></placeName></affiliation></author></analytic><monogr></monogr><series><title level="j">Acta Crystallographica Section D</title><title level="j" type="abbrev">Acta Cryst. D</title><idno type="ISSN">1399-0047</idno><idno type="eISSN">1399-0047</idno><imprint><publisher>Munksgaard International Publishers</publisher><pubPlace>5 Abbey Square, Chester, Cheshire CH1 2HU, England</pubPlace><date type="published" when="2004-11">2004-11</date><biblScope unit="volume">60</biblScope><biblScope unit="issue">11</biblScope><biblScope unit="page" from="2107">2107</biblScope><biblScope unit="page" to="2109">2109</biblScope></imprint><idno type="ISSN">1399-0047</idno></series><idno type="istex">8DAD7EA4E8046AC3148EB40846CE1E985B53AC5E</idno><idno type="DOI">10.1107/S0907444904024229</idno><idno type="ArticleID">AYDTS5035</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">1399-0047</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>detergent</term><term>flap stabilization.</term><term>lipase</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Horse pancreatic lipase‐related proteins PLRP1 and PLRP2 are produced by the pancreas together with pancreatic lipase (PL). Sequence‐comparison analyses reveal that the three proteins possess the same two‐domain organization: an N‐terminal catalytic domain and a C‐terminal domain, which in PL is involved in colipase binding. Nevertheless, despite the high level of sequence identity found, they exhibit distinct enzymatic properties. The intrinsic sensitivity of the peptide bond between Ser245 and Thr246 within the flap region of PLRP2 to proteolytic cleavage probably complicates PLRP2 crystallization since, as shown here, this proteolyzed form of PLRP2 is only crystallized after specific detergent stabilization of this region. This has been performed by the hanging‐drop vapour‐diffusion method at 291 K and exclusively in the presence of N,N‐dimethyldecylamine‐β‐oxide (DDAO). However, most crystals (>95%) are highly twinned and diffract poorly (to ∼7–5 Å resolution). Diffraction‐quality trigonal crystals have unit‐cell parameters a = b = 128.4, c = 85.8 Å and belong to space group P3221. A 2.9 Å native data set was collected at ESRF on beamline ID14‐2 with an Rmerge of 12.7%. Preliminary structural analysis provides a structural basis for the specific roles of DDAO.</div></front></TEI><affiliations><list><country><li>Espagne</li><li>France</li></country><region><li>Communauté de Madrid</li><li>Provence-Alpes-Côte d'Azur</li></region><settlement><li>Marseille</li></settlement></list><tree><country name="Espagne"><region name="Communauté de Madrid"><name sortKey="Manche O, Jose M" sort="Manche O, Jose M" uniqKey="Manche O J" first="José M." last="Manche O">José M. Manche O</name></region><name sortKey="Chapus, Catherine" sort="Chapus, Catherine" uniqKey="Chapus C" first="Catherine" last="Chapus">Catherine Chapus</name><name sortKey="Crenon, Isabelle" sort="Crenon, Isabelle" uniqKey="Crenon I" first="Isabelle" last="Crenon">Isabelle Crenon</name><name sortKey="Hermoso, Juan A" sort="Hermoso, Juan A" uniqKey="Hermoso J" first="Juan A." last="Hermoso">Juan A. Hermoso</name><name sortKey="Jayne, Sandrine" sort="Jayne, Sandrine" uniqKey="Jayne S" first="Sandrine" last="Jayne">Sandrine Jayne</name><name sortKey="Kerfelec, Brigritte" sort="Kerfelec, Brigritte" uniqKey="Kerfelec B" first="Brigritte" last="Kerfelec">Brigritte Kerfelec</name></country><country name="France"><region name="Provence-Alpes-Côte d'Azur"><name sortKey="Manche O, Jose M" sort="Manche O, Jose M" uniqKey="Manche O J" first="José M." last="Manche O">José M. Manche O</name></region><name sortKey="Chapus, Catherine" sort="Chapus, Catherine" uniqKey="Chapus C" first="Catherine" last="Chapus">Catherine Chapus</name><name sortKey="Crenon, Isabelle" sort="Crenon, Isabelle" uniqKey="Crenon I" first="Isabelle" last="Crenon">Isabelle Crenon</name><name sortKey="Hermoso, Juan A" sort="Hermoso, Juan A" uniqKey="Hermoso J" first="Juan A." last="Hermoso">Juan A. Hermoso</name><name sortKey="Jayne, Sandrine" sort="Jayne, Sandrine" uniqKey="Jayne S" first="Sandrine" last="Jayne">Sandrine Jayne</name><name sortKey="Kerfelec, Brigritte" sort="Kerfelec, Brigritte" uniqKey="Kerfelec B" first="Brigritte" last="Kerfelec">Brigritte Kerfelec</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Musique/explor/MozartV1/Data/France/Analysis
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000079 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/France/Analysis/biblio.hfd -nk 000079 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Musique
|area= MozartV1
|flux= France
|étape= Analysis
|type= RBID
|clé= ISTEX:8DAD7EA4E8046AC3148EB40846CE1E985B53AC5E
|texte= Crystallization of a proteolyzed form of the horse pancreatic lipase‐related protein 2: structural basis for the specific detergent requirement
}}
| This area was generated with Dilib version V0.6.20. |  |